BIOL 601 - Molecular Biotechnology

Six hours.
4 Credits

This is predominantly a laboratory course with direct hands-on laboratory experiences using state-of-the-art techniques and experimental approaches in the production of heterologous proteins in prokaryotic and eukaryotic cells utilizing bacterial (prokaryotic) as well as insect, yeast, and mammalian (eukaryotic) expression vectors. Students will use molecular biology approaches, including techniques in recombinant DNA and genetic engineering technology to clone, express, affinity-purify, and characterize the recombinant proteins produced in the prokaryotic and eukaryotic host cells. The theoretical component of the course introduces the student to the fundamental principles, applications, strategies, and societal concerns of Molecular Biotechnology, and will facilitate an understanding of important theoretical concepts which will be complemented by the methodologies and experimental strategies covered in the laboratory portion of the course.